Major proteinase movement upon stable serpin-proteinase complex formation
نویسندگان
چکیده
منابع مشابه
Formation of the covalent serpin-proteinase complex involves translocation of the proteinase by more than 70 Å and full insertion of the reactive center loop into b-sheet A (a1-proteinase inhibitoryPittsburgh variantyserpin covalent complexyf luorescence resonance energy transfer)
To determine the location of the proteinase in the covalent serpin-proteinase complex we prepared seven single-cysteine-containing variants of the Pittsburgh variant of the serpin a1-proteinase inhibitor, and we labeled each cysteine with the dansyl f luorophore. The dansyl probes were used to determine proximity of the proteinase trypsin in covalent and noncovalent complexes with the serpin, b...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.2.453